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MED10 Polyclonal antibody

MED10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15196-1-AP

Synonyms

L6, MED10, mediator complex subunit 10, NUT2, TRG 17, TRG 20, TRG17, TRG20

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using MED10 Polyclonal antibody (15196-1-AP)

    WB analysis of COLO 320 using 15196-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15196-1-AP (MED10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15196-1-AP targets MED10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MED10 fusion protein Ag7336
Full Name mediator complex subunit 10
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC003353
Gene Symbol MED10
Gene ID (NCBI) 84246
RRIDAB_2878117
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED10, also named as L6, TRG17, or TRG20, is a 135 amino acid protein, which belongs to the Mediator complex subunit 10 family. MED10 localizes in the nucleus and as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Multiprotein mediator complex acting as a coactivator is required for activation of RNA polymerase II transcription by DNA bound transcription factors.

Protocols

Product Specific Protocols
WB protocol for MED10 antibody 15196-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex.

Authors - Vijayalingam S S
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

SITING (Verified Customer) (04-02-2021)

can see band aroun 15kd

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: EMBRYO HEART
MED10 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:15196-1-AP)