- Featured Product
- KD/KO Validated
MCRS1 Polyclonal antibody
MCRS1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, CoIP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 11362-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, human brain tissue, human liver tissue, human spleen tissue, mouse spleen tissue, mouse uterus tissue, rat spleen tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
11362-1-AP targets MCRS1 in WB, IF, IHC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MCRS1 fusion protein Ag1904 |
| Full Name | microspherule protein 1 |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC011794 |
| Gene Symbol | MCRS1 |
| Gene ID (NCBI) | 10445 |
| RRID | AB_2143116 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MCRS1, also named INO80Q, MSP58 or p78, is a nucleolar protein expressed in the very early S phase. It modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. MCRS1 is also present on polyribosomes of synaptoneurosome as a novel RNA-binding protein to activate the rRNA transcription. The forkhead-associated domain of MCRS1 is involved in association with centrosomal protein Nde1, comprising a component of the centrosome and taking an essential role in viability. Its isoform MCRS2 might be a linker between telomere maintenance and cell-cycle regulation by interacting with LPTS/PinX1, a telomerase-inhibitory protein. MCRS1 is detected by Western blot as a highly modified protein at 78 kDa and minor bands at about 62 and 55 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MCRS1 antibody 11362-1-AP | Download protocol |
| IHC protocol for MCRS1 antibody 11362-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Rep Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis
| ||
Sci Rep MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment. | ||
J Biol Chem Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. | ||
Mol Biol Cell Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment. |
