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MCM7 Polyclonal antibody

MCM7 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11225-1-AP

Synonyms

P1.1 MCM3, MCM2, EC:3.6.4.12, CDC47 homolog, CDC47

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCM7 Polyclonal antibody (11225-1-AP)

    WB analysis using 11225-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11225-1-AP (MCM7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using MCM7 Polyclonal antibody (11225-1-AP)

    WB analysis of NIH/3T3 using 11225-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11225-1-AP (MCM7 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MCM7 Polyclonal antibody (11225-1-AP)

    WB analysis of MCF-7 using 11225-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11225-1-AP (MCM7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using MCM7 Polyclonal antibody (11225-1-AP)

    IP experiment of NIH/3T3 using 11225-1-AP

    IP result of anti-MCM7 (IP:11225-1-AP, 4ug; Detection:11225-1-AP 1:2000) with NIH/3T3 cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MCM7 Polyclonal antibody (11225-1-AP)

    IHC staining of human lung cancer using 11225-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MCM7 Polyclonal antibody (11225-1-AP)

    IHC staining of human lung cancer using 11225-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using MCM7 Polyclonal antibody (11225-1-AP)

    IHC staining of human lymphoma using 11225-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using MCM7 Polyclonal antibody (11225-1-AP)

    IHC staining of human lymphoma using 11225-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11225-1-AP (MCM7 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MCM7 Polyclonal antibody (11225-1-AP)

    IHC staining of human lung cancer using 11225-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MCM7 Polyclonal antibody (11225-1-AP)

    IHC staining of human lung cancer using 11225-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MCM7 Polyclonal antibody (11225-1-AP)

    IHC staining of human lung cancer using 11225-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MCM7 Polyclonal antibody (11225-1-AP)

    IF Staining of A431 using 11225-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MCM7 antibody (11225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using MCM7 Polyclonal antibody (11225-1-AP)

    FC experiment of HepG2 using 11225-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MCM7 (11225-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, NIH/3T3 cells, Jurkat cells, K-562 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11225-1-AP targets MCM7 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCM7 fusion protein Ag1730
Full Name minichromosome maintenance complex component 7
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC013375
Gene Symbol MCM7
Gene ID (NCBI) 4176
RRIDAB_2297584
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2-7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].

Protocols

Product Specific Protocols
WB protocol for MCM7 antibody 11225-1-APDownload protocol
IHC protocol for MCM7 antibody 11225-1-APDownload protocol
IF protocol for MCM7 antibody 11225-1-APDownload protocol
IP protocol for MCM7 antibody 11225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer

Authors - Huaiyuan Liang
View Article
humanWB

Nucleic Acids Res

DOCK7 protects against replication stress by promoting RPA stability on chromatin.

Authors - Ming Gao
View Article
humanWB

Biomaterials

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Authors - Hongyuan Song
View Article
humanWB

Elife

The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP

Authors - Maria Körner
View Article
humanWB

Elife

MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.

Authors - Yuichiro Saito
View Article
humanWB

J Nanobiotechnology

Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling.

Authors - Na Zhao
View Article