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- KD/KO Validated
MCM3 Polyclonal antibody
MCM3 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15597-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, Raji cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 14 publications below |
| IHC | See 5 publications below |
Product Information
15597-1-AP targets MCM3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MCM3 fusion protein Ag7966 |
| Full Name | minichromosome maintenance complex component 3 |
| Calculated Molecular Weight | 91 kDa |
| Observed Molecular Weight | 102-105 kDa |
| GenBank Accession Number | BC001626 |
| Gene Symbol | MCM3 |
| Gene ID (NCBI) | 4172 |
| RRID | AB_2141973 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). MCM3 has 2 isoforms with the molecular mass of 91 and 96 kDa. The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MCM3 antibody 15597-1-AP | Download protocol |
| IHC protocol for MCM3 antibody 15597-1-AP | Download protocol |
| IP protocol for MCM3 antibody 15597-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer. | ||
Biomaterials Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis. | ||
Aging Cell Pyrroloquinoline quinone alleviates natural aging-related osteoporosis via a novel MCM3-Keap1-Nrf2 axis-mediated stress response and Fbn1 upregulation
| ||
Elife MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells. | ||
Oncotarget Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis. | ||
Ecotoxicol Environ Saf The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis. |
