• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

MCL1 Polyclonal antibody

MCL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 16225-1-AP

Synonyms

Mcl 1, Induced myeloid leukemia cell differentiation protein Mcl-1, EAT, Bcl-2-related protein EAT/mcl1, Bcl-2-like protein 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MCL1 Polyclonal antibody (16225-1-AP)

    WB analysis using 16225-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MCL1 Polyclonal antibody (16225-1-AP)

    WB analysis using 16225-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using MCL1 Polyclonal antibody (16225-1-AP)

    WB analysis of Raji using 16225-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using MCL1 Polyclonal antibody (16225-1-AP)

    WB analysis of HL-60 using 16225-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MCL1 Polyclonal antibody (16225-1-AP)

    WB analysis using 16225-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using MCL1 Polyclonal antibody (16225-1-AP)

    WB analysis of mouse spleen using 16225-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using MCL1 Polyclonal antibody (16225-1-AP)

    WB analysis of A431 using 16225-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MCL1 Polyclonal antibody (16225-1-AP)

    WB analysis of K-562 using 16225-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using MCL1 Polyclonal antibody (16225-1-AP)

    WB analysis of human liver using 16225-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MCL1 Polyclonal antibody (16225-1-AP)

    WB analysis of human brain using 16225-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using MCL1 Polyclonal antibody (16225-1-AP)

    IP experiment of Raji using 16225-1-AP

    IP result of anti-MCL1 (IP:16225-1-AP, 3ug; Detection:16225-1-AP 1:1000) with Raji cells lysate 800ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MCL1 Polyclonal antibody (16225-1-AP)

    IHC staining of human ovary cancer using 16225-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16225-1-AP (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MCL1 Polyclonal antibody (16225-1-AP)

    IHC staining of human ovary cancer using 16225-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16225-1-AP (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MCL1 Polyclonal antibody (16225-1-AP)

    IF Staining of MCF-7 using 16225-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MCL1 antibody (16225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of Ramos cells using MCL1 Polyclonal antibody (16225-1-AP)

    FC experiment of Ramos using 16225-1-AP

    1X10^6 Ramos cells were intracellularly stained with 0.5 ug Anti-Human MCL1 (16225-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inJurkat cells, mouse spleen tissue, Raji cells, HL-60 cells, A431 cells, human liver tissue, HEK-293T cells, human brain tissue, K-562 cells, MCF-7 cells, HeLa cells
Positive IP detected inRaji cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16225-1-AP targets MCL1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCL1 fusion protein Ag10397
Full Name myeloid cell leukemia sequence 1 (BCL2-related)
Calculated Molecular Weight 350 aa, 37 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC107735
Gene Symbol MCL1
Gene ID (NCBI) 4170
RRIDAB_2143977
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. MCL-1 can be alternatively spliced into a long form (MCL-1L, 40 kDa) or a short form (MCL-1S, 30 kDa). (PMID: 15467463, PMID: 15147382)

Protocols

Product Specific Protocols
WB protocol for MCL1 antibody 16225-1-APDownload protocol
IHC protocol for MCL1 antibody 16225-1-APDownload protocol
IF protocol for MCL1 antibody 16225-1-APDownload protocol
IP protocol for MCL1 antibody 16225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nat Commun

MCL-1 gains occur with high frequency in lung adenocarcinoma and can be targeted therapeutically.

Authors - Enkhtsetseg Munkhbaatar
View Article
humanWB

Nat Commun

Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease.

Authors - Di Hu
View Article
mouseWB

Nat Commun

IFN-β is a macrophage-derived effector cytokine facilitating the resolution of bacterial inflammation.

Authors - Senthil Kumaran Satyanarayanan
View Article
humanWB

EBioMedicine

The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis.

Authors - Pingbo Chen
View Article
humanWB,IP

Nat Commun

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Authors - Xin Li
View Article
humanWB

Aging (Albany NY)

Melatonin induces cell cycle arrest and suppresses tumor invasion in urinary bladder urothelial carcinoma

Authors - Tzuo-Yi Hsieh
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Eya (Verified Customer) (07-06-2021)

the bands appear very clear

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: shTHP1