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MBTD1 Polyclonal antibody

MBTD1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26098-1-AP

Synonyms

mbt domain containing 1, MBTD1, SA49P01

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using MBTD1 Polyclonal antibody (26098-1-AP)

    WB analysis of Jurkat using 26098-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26098-1-AP (MBTD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using MBTD1 Polyclonal antibody (26098-1-AP)

    WB analysis of mouse spleen using 26098-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26098-1-AP (MBTD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using MBTD1 Polyclonal antibody (26098-1-AP)

    WB analysis of HL-60 using 26098-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26098-1-AP (MBTD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MBTD1 Polyclonal antibody (26098-1-AP)

    WB analysis of K-562 using 26098-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 26098-1-AP (MBTD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using MBTD1 Polyclonal antibody (26098-1-AP)

    IF Staining of U-251 using 26098-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using MBTD1 antibody (26098-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, mouse spleen tissue, HL-60 cells
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 26098-1-AP is MBTD1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MBTD1 fusion protein Ag22059
Full Name mbt domain containing 1
Calculated Molecular Weight 628 aa, 70 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC101736
Gene Symbol MBTD1
Gene ID (NCBI) 54799
RRIDAB_2880374
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MBTD1 antibody 26098-1-APDownload protocol
IF protocol for MBTD1 antibody 26098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta Mol Basis Dis

BAP18 acting as a novel peroxisome proliferator-activated receptor α co-regulator contributes to hepatocellular carcinoma progression

Authors - Wei Liu
View Article