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  • Featured Product
  • KD/KO Validated

MBD4 Polyclonal antibody

MBD4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11270-1-AP

Synonyms

MBD4, MED1

Validation Data Gallery

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  • Western Blot (WB) analysis of human skin tissue using MBD4 Polyclonal antibody (11270-1-AP)

    WB analysis of human skin using 11270-1-AP

    human skin tissue were subjected to SDS PAGE followed by western blot with 11270-1-AP (MBD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using MBD4 Polyclonal antibody (11270-1-AP)

    WB analysis of A549 using 11270-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11270-1-AP (MBD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman skin tissue, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11270-1-AP targets MBD4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MBD4 fusion protein Ag1822
Full Name methyl-CpG binding domain protein 4
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC011752
Gene Symbol MBD4
Gene ID (NCBI) 8930
RRIDAB_2139871
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBD4, also named as MED1, is a 580 amino acid protein, which Interacts with MLH1. MBD4 as a mismatch-specific DNA N-glycosylase is involved in DNA repair and has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. MBD4 can also remove uracil or 5-fluorouracil in G:U mismatches. MBD4 exists some isoforms with MV 66 kDa and 30 kDa (The identification of a novel alternatively spliced form of the MBD4 DNA glycosylase).

Protocols

Product Specific Protocols
WB protocol for MBD4 antibody 11270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
  • KD Validated
View Article
humanWB

Genet Test Mol Biomarkers

The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.

Authors - Mei-Rong Liang
View Article