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MATR3 Polyclonal antibody

MATR3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12202-2-AP

Synonyms

Matrin-3, matrin 3, KIAA0723

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using MATR3 Polyclonal antibody (12202-2-AP)

    WB analysis of MCF-7 using 12202-2-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12202-2-AP (MATR3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MATR3 Polyclonal antibody (12202-2-AP)

    IHC staining of human lung cancer using 12202-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12202-2-AP (MATR3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MATR3 Polyclonal antibody (12202-2-AP)

    IHC staining of mouse testis using 12202-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12202-2-AP (MATR3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MATR3 Polyclonal antibody (12202-2-AP)

    IHC staining of human lung cancer using 12202-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12202-2-AP (MATR3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using MATR3 Polyclonal antibody (12202-2-AP)

    IF Staining of SH-SY5Y using 12202-2-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using MATR3 antibody (12202-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MATR3 Polyclonal antibody (12202-2-AP)

    IF Staining of HEK-293 using 12202-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MATR3 antibody (12202-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MATR3 Polyclonal antibody (12202-2-AP)

    IF Staining of HEK-293 using 12202-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MATR3 antibody (12202-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12202-2-AP targets MATR3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MATR3 fusion protein Ag2843
Full Name matrin 3
Calculated Molecular Weight 847 aa, 95 kDa
Observed Molecular Weight125 kDa
GenBank Accession NumberBC015031
Gene Symbol MATR3
Gene ID (NCBI) 9782
RRIDAB_2281752
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MATR3, known as KIAA0723, is an 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa protein as a result of PMID: 28977530.

Protocols

Product Specific Protocols
WB protocol for MATR3 antibody 12202-2-APDownload protocol
IHC protocol for MATR3 antibody 12202-2-APDownload protocol
IF protocol for MATR3 antibody 12202-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Authors - Roy Matkovic
View Article
humanWB

Nat Commun

An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis.

Authors - Song Zhu
View Article
humanWB

Brain

MATR3 haploinsufficiency and early-onset neurodegeneration.

Authors - Michael Zech
View Article
humanWB

J Cell Biol

Stress granules regulate stress-induced paraspeckle assembly.

Authors - Haiyan An
View Article
humanWB

Cell Rep

SATB1 regulates 3D genome architecture in T cells by constraining chromatin interactions surrounding CTCF-binding sites

Authors - Bao Wang
View Article
humanWB

RNA Biol

Modulation of PDCD1 exon 3 splicing.

Authors - Junjie Sun
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Roy (Verified Customer) (06-12-2024)

Strong MATR3 band detected with a 1/1000 dilution in HeLa and Jurkat cells (1h incubation at RT)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa, Jurkat
FH

Tatyana (Verified Customer) (03-11-2023)

Good and specific nuclear staining. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h at RT incubation.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human, SH-SY5Y cells
MATR3 Antibody Immunofluorescence validation (1:1000 dilution) in Human, SH-SY5Y cells (Cat no:12202-2-AP)
FH

Wael (Verified Customer) (08-22-2022)

around 50ug of total cell lysate Blocking: 5% skim milk in PBS Primary incubation: 1:1000 in 5% skim milk in PBS overnight, rotating 4C Washes : 3X 15mins of PBS(0.001% Tween) Secondary: 1:10000 in 5% skim milk in PBS, 1hr, RT Washes : 3X 15mins of PBS(0.001% Tween) Specific Band size around 100KD

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293
MATR3 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:12202-2-AP)