• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MATK Polyclonal antibody

MATK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10082-2-AP

Synonyms

Tyrosine-protein kinase CTK, Megakaryocyte-associated tyrosine-protein kinase, HYL, Hematopoietic consensus tyrosine-lacking kinase, EC:2.7.10.2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of K-562 cells using MATK Polyclonal antibody (10082-2-AP)

    WB analysis of K-562 using 10082-2-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10082-2-AP (MATK antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using MATK Polyclonal antibody (10082-2-AP)

    WB analysis of mouse thymus using 10082-2-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10082-2-AP (MATK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MATK Polyclonal antibody (10082-2-AP)

    IHC staining of human breast cancer using 10082-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10082-2-AP (MATK Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MATK Polyclonal antibody (10082-2-AP)

    IHC staining of human breast cancer using 10082-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10082-2-AP (MATK Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MATK Polyclonal antibody (10082-2-AP)

    IF Staining of HepG2 using 10082-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MATK antibody (10082-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, mouse thymus tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

10082-2-AP targets MATK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MATK fusion protein Ag0128
Full Name megakaryocyte-associated tyrosine kinase
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC000114
Gene Symbol MATK
Gene ID (NCBI) 4145
RRIDAB_2141386
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MATK(Megakaryocyte-associated tyrosine-protein kinase) is also named as CTK, HYL and belongs to the protein kinase superfamily. This protein can can inhibit the kinase activity of certain Src kinase family members by phosphorylating the C-terminal tyrosine and by a non-catalytic mechanism. It may play an inhibitory role in the control of T-cell proliferation. MATK has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MATK antibody 10082-2-APDownload protocol
IHC protocol for MATK antibody 10082-2-APDownload protocol
IF protocol for MATK antibody 10082-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Biomed Pharmacother

Anti-osteoporotic effects of alisol C 23-acetate via osteoclastogenesis inhibition.

Authors - Xiaokang Jia
View Article
humanWB

Biophys Rep

Detection of ribophagy in yeast and mammals

Authors - Miao Ye
View Article