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MARCKSL1 Polyclonal antibody

MARCKSL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14124-1-AP

Synonyms

MARCKS like protein 1, MARCKS like 1, Macrophage myristoylated alanine-rich C kinase substrate, Mac-MARCKS, MacMARCKS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MARCKSL1 Polyclonal antibody (14124-1-AP)

    WB analysis using 14124-1-AP

    Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14124-1-AP (MARCKSL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MARCKSL1 Polyclonal antibody (14124-1-AP)

    WB analysis of human brain using 14124-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14124-1-AP (MARCKSL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MARCKSL1 Polyclonal antibody (14124-1-AP)

    WB analysis of human brain using 14124-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14124-1-AP (MARCKSL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of skin cancer using MARCKSL1 Polyclonal antibody (14124-1-AP)

    IHC staining of skin cancer using 14124-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14124-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue
Positive IHC detected inskin cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14124-1-AP targets MARCKSL1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MARCKSL1 fusion protein Ag5274
Full Name MARCKS-like 1
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC066915
Gene Symbol MARCKSL1
Gene ID (NCBI) 65108
RRIDAB_2140454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARCKS-like protein 1 (MARCKSL1) is widely expressed in nervous tissue, it is also named MARCKS-like protein (MLP) or MARCKS-related protein (MRP) and belongs to the MARCKS family, which is a highly acidic myristoylated family of PKC substrates widely distributed in diverse cell types including macrophages. The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation. Genetic disruption of MARCKSL1 results in neural tube closure defects, events that depend on coordinated control of actin functions, cell shape, and cell migration. MARCKSL1 are thought to regulate the actin cytoskeleton and thereby participate in major cellular responses such as phagocytosis, secretion, motility, mitogenesis, and membrane trafficking.

Protocols

Product Specific Protocols
WB protocol for MARCKSL1 antibody 14124-1-APDownload protocol
IHC protocol for MARCKSL1 antibody 14124-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols