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MARCKSL1 Polyclonal antibody

MARCKSL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11422-1-AP

Synonyms

F52, Mac MARCKS, MACMARCKS, MARCKS like 1, MARCKS like protein 1, MARCKS related protein, MARCKSL1, MLP, MLP1, MRP

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using MARCKSL1 Polyclonal antibody (11422-1-AP)

    WB analysis of human brain using 11422-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11422-1-AP (MARCKSL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MARCKSL1 Polyclonal antibody (11422-1-AP)

    WB analysis of human brain using 11422-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11422-1-AP (MARCKSL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MARCKSL1 Polyclonal antibody (11422-1-AP)

    IHC staining of human lung cancer using 11422-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11422-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MARCKSL1 Polyclonal antibody (11422-1-AP)

    IHC staining of human lung cancer using 11422-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11422-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MARCKSL1 Polyclonal antibody (11422-1-AP)

    IHC staining of human breast cancer using 11422-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11422-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MARCKSL1 Polyclonal antibody (11422-1-AP)

    IHC staining of human breast cancer using 11422-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11422-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11422-1-AP targets MARCKSL1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MARCKSL1 fusion protein Ag1967
Full Name MARCKS-like 1
Calculated Molecular Weight 195 aa, 20 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC007904
Gene Symbol MARCKSL1
Gene ID (NCBI) 65108
RRIDAB_2140456
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARCKS-like protein 1 (MARCKSL1) is widely expressed in nervous tissue, it is also named MARCKS-like protein (MLP) or MARCKS-related protein (MRP) and belongs to the MARCKS family, which is a highly acidic myristoylated family of PKC substrates widely distributed in diverse cell types including macrophages. The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation. Genetic disruption of MARCKSL1 results in neural tube closure defects, events that depend on coordinated control of actin functions, cell shape, and cell migration. MARCKSL1 are thought to regulate the actin cytoskeleton and thereby participate in major cellular responses such as phagocytosis, secretion, motility, mitogenesis, and membrane trafficking.

Protocols

Product Specific Protocols
WB protocol for MARCKSL1 antibody 11422-1-APDownload protocol
IHC protocol for MARCKSL1 antibody 11422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Proteome Res

Sparc-like protein 1 is a new marker of human glioma progression.

Authors - Turtoi Andrei A
View Article