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  • Featured Product
  • KD/KO Validated

MAPKAPK5 Polyclonal antibody

MAPKAPK5 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14339-1-AP

Synonyms

MAPKAP-K5, MAPKAPK 5, MAPKAP kinase 5, MAPK-activated protein kinase 5, MAP kinase-activated protein kinase 5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MAPKAPK5 Polyclonal antibody (14339-1-AP)

    WB analysis of HeLa using 14339-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MAPKAPK5 Polyclonal antibody (14339-1-AP)

    WB analysis of Jurkat using 14339-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAPKAPK5 Polyclonal antibody (14339-1-AP)

    IF Staining of HeLa using 14339-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14339-1-AP targets MAPKAPK5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAPKAPK5 fusion protein Ag5650
Full Name mitogen-activated protein kinase-activated protein kinase 5
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC047284
Gene Symbol MAPKAPK5
Gene ID (NCBI) 8550
RRIDAB_2141473
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAPKAPK5(MAP kinase-activated protein kinase 5) is also named as PRAK and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK5 contains the conserved protein kinase domains I through XI, which are characteristic of all protein kinases. It is a major stress-activated kinase that can phosphorylate small heat shock protein, suggesting a potential role for PRAK in mediating stress-induced HSP27 phosphorylation in vivo(PMID:9628874). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MAPKAPK5 antibody 14339-1-APDownload protocol
IF protocol for MAPKAPK5 antibody 14339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Nat Commun

The essential role of PRAK in tumor metastasis and its therapeutic potential.

Authors - Yuqing Wang
  • KO Validated
View Article
humanWB, IP

J Cancer Biol Res

Cross-Phosphorylation and Interaction between Src/FAK and MAPKAP5/PRAK in Early Focal Adhesions Controls Cell Motility.

Authors - Sheila Figel Dwyer
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

hala (Verified Customer) (06-16-2022)

it works very good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: hepG2