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MAP9 Polyclonal antibody

MAP9 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26078-1-AP

Synonyms

Microtubule-associated protein 9, Aster-associated protein, Aster associated protein, ASAP

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using MAP9 Polyclonal antibody (26078-1-AP)

    WB analysis of SH-SY5Y using 26078-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 26078-1-AP (MAP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MAP9 Polyclonal antibody (26078-1-AP)

    WB analysis of HeLa using 26078-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26078-1-AP (MAP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MAP9 Polyclonal antibody (26078-1-AP)

    WB analysis of K-562 using 26078-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 26078-1-AP (MAP9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAP9 Polyclonal antibody (26078-1-AP)

    IF Staining of HeLa using 26078-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MAP9 antibody (26078-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inSH-SY5Y cells, HeLa cells, K-562 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26078-1-AP targets MAP9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP9 fusion protein Ag23446
Full Name microtubule-associated protein 9
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC146864
Gene Symbol MAP9
Gene ID (NCBI) 79884
RRIDAB_2880366
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAP9 antibody 26078-1-APDownload protocol
IF protocol for MAP9 antibody 26078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canineIF

Front Cell Neurosci

Molecular characterization of MAP9 in the photoreceptor sensory cilia as a modifier in canine RPGRIP1-associated cone-rod dystrophy

Authors - Kei Takahashi
View Article
humanIHC,IF

Dev Cell

MAP9/MAPH-9 supports axonemal microtubule doublets and modulates motor movement

Authors - Michael V Tran
View Article