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MAP4K5 Polyclonal antibody

MAP4K5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12827-1-AP

Synonyms

GCKR, KHS, KHS1, MAP4K5, MAPKKKK5, MEK kinase kinase 5, MEKKK 5

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using MAP4K5 Polyclonal antibody (12827-1-AP)

    WB analysis of mouse ovary using 12827-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12827-1-AP (MAP4K5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MAP4K5 Polyclonal antibody (12827-1-AP)

    WB analysis of HeLa using 12827-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12827-1-AP (MAP4K5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MAP4K5 Polyclonal antibody (12827-1-AP)

    WB analysis of K-562 using 12827-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12827-1-AP (MAP4K5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using MAP4K5 Polyclonal antibody (12827-1-AP)

    WB analysis of RAW264.7 using 12827-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 12827-1-AP (MAP4K5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using MAP4K5 Polyclonal antibody (12827-1-AP)

    IHC staining of human ovary tumor using 12827-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12827-1-AP (MAP4K5 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inmouse ovary tissue, HeLa cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12827-1-AP targets MAP4K5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP4K5 fusion protein Ag3547
Full Name mitogen-activated protein kinase kinase kinase kinase 5
Calculated Molecular Weight 846 aa, 95 kDa
Observed Molecular Weight 58 kDa, 95 kDa, 120 kDa
GenBank Accession NumberBC036013
Gene Symbol MAP4K5
Gene ID (NCBI) 11183
RRIDAB_2140986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAP4K5 antibody 12827-1-APDownload protocol
IHC protocol for MAP4K5 antibody 12827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols