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MAP3K4 Polyclonal antibody

MAP3K4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21610-1-AP

Synonyms

KIAA0213, MAP three kinase 1, MAP3K4, MAPK/ERK kinase kinase 4, MAPKKK4, MEK kinase 4, MEKK 4, MEKK4, MTK1, PRO0412

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using MAP3K4 Polyclonal antibody (21610-1-AP)

    WB analysis of K-562 using 21610-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 21610-1-AP (MAP3K4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MAP3K4 Polyclonal antibody (21610-1-AP)

    WB analysis of K-562 using 21610-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 21610-1-AP (MAP3K4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAP3K4 Polyclonal antibody (21610-1-AP)

    IF Staining of HeLa using 21610-1-AP

    Immunofluorescent analysis of Hela cells, using MAP3K4 antibody 21610-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inK-562 cells
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 21610-1-AP is MAP3K4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP3K4 fusion protein Ag16254
Full Name mitogen-activated protein kinase kinase kinase 4
Calculated Molecular Weight 1608 aa, 182 kDa
Observed Molecular Weight 170-180 kDa
GenBank Accession NumberBC146770
Gene Symbol MAP3K4
Gene ID (NCBI) 4216
RRIDAB_10804775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAP3K4 antibody 21610-1-APDownload protocol
IF protocol for MAP3K4 antibody 21610-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Gene Med

Construction and validation of molecular subtype and signature of immune cell-related telomeric genes and prediction of prognosis and immunotherapy efficacy in ovarian cancer patients

Authors - Lele Ling
View Article