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MANEA Polyclonal antibody

MANEA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21296-1-AP

Synonyms

ENDO, Endo alpha mannosidase, Endomannosidase, hEndo, Mandaselin, MANEA, mannosidase, endo alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MANEA Polyclonal antibody (21296-1-AP)

    WB analysis of HEK-293 using 21296-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21296-1-AP (MANEA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using MANEA Polyclonal antibody (21296-1-AP)

    WB analysis of mouse kidney using 21296-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21296-1-AP (MANEA Antibody) at dilution of 1:100 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MANEA Polyclonal antibody (21296-1-AP)

    IHC staining of human breast cancer using 21296-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MANEA Polyclonal antibody (21296-1-AP)

    IHC staining of human breast cancer using 21296-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MANEA Polyclonal antibody (21296-1-AP)

    IF Staining of HEK-293 using 21296-1-AP

    Immunofluorescent analysis of HEK-293 cells using 21296-1-AP (MANEA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21296-1-AP is MANEA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MANEA fusion protein Ag15847
Full Name mannosidase, endo-alpha
Calculated Molecular Weight 462 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC146671
Gene Symbol MANEA
Gene ID (NCBI) 79694
RRIDAB_2878836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MANEA antibody 21296-1-APDownload protocol
IHC protocol for MANEA antibody 21296-1-APDownload protocol
IF protocol for MANEA antibody 21296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols