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MANBAL Polyclonal antibody

MANBAL Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16028-1-AP

Synonyms

Protein MANBAL

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human brain tissue using MANBAL Polyclonal antibody (16028-1-AP)

    IHC staining of human brain using 16028-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16028-1-AP (MANBAL antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MANBAL Polyclonal antibody (16028-1-AP)

    IHC staining of human brain using 16028-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16028-1-AP (MANBAL antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MANBAL Polyclonal antibody (16028-1-AP)

    IF Staining of U2OS using 16028-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MANBAL antibody (16028-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16028-1-AP targets MANBAL in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MANBAL fusion protein Ag8888
Full Name mannosidase, beta A, lysosomal-like
Calculated Molecular Weight 85 aa, 10 kDa
GenBank Accession NumberBC014672
Gene Symbol MANBAL
Gene ID (NCBI) 63905
RRIDAB_2139582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for MANBAL antibody 16028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols