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MAML3 Polyclonal antibody

MAML3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23263-1-AP

Synonyms

CAGH3, ERDA3, GDN, KIAA1816, MAM 2, Mam 3, MAM2, MAML3, mastermind like 3 (Drosophila), Mastermind like protein 3, TNRC3

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MAML3 Polyclonal antibody (23263-1-AP)

    WB analysis of HEK-293 using 23263-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23263-1-AP (MAML3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MAML3 Polyclonal antibody (23263-1-AP)

    WB analysis of HEK-293 using 23263-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23263-1-AP (MAML3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MAML3 Polyclonal antibody (23263-1-AP)

    IF Staining of HEK-293 using 23263-1-AP

    Immunofluorescent analysis of HEK-293 cells using 23263-1-AP (MAML3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23263-1-AP is MAML3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAML3 fusion protein Ag19788
Full Name mastermind-like 3 (Drosophila)
Calculated Molecular Weight 1133 aa, 122 kDa
Observed Molecular Weight 122 kDa
GenBank Accession NumberBC137131
Gene Symbol MAML3
Gene ID (NCBI) 55534
RRIDAB_2879242
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The three MAML genes are widely expressed in adult tissues but exhibit distinct expression patterns in mouse early spinal cord development. All MAML proteins localize to nuclear bodies, share a conserved basic domain in their N termini that binds to the ankyrin repeat domain of Notch, and contain a transcriptional activation domain in their C termini. MAML3 acts as a transcriptional coactivator for NOTCH proteins and has been shown to amplify NOTCH-induced transcription of HES1.

Protocols

Product Specific Protocols
WB protocol for MAML3 antibody 23263-1-APDownload protocol
IF protocol for MAML3 antibody 23263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols