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MAK16 Polyclonal antibody

MAK16 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17505-1-AP

Synonyms

MAK16, MAK16 homolog (S. cerevisiae), MAK16L, NNP78, Protein MAK16 homolog, Protein RBM13, RBM13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAK16 Polyclonal antibody (17505-1-AP)

    WB analysis using 17505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17505-1-AP (MAK16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MAK16 Polyclonal antibody (17505-1-AP)

    IF Staining of HEK-293 using 17505-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 17505-1-AP (MAK16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17505-1-AP targets MAK16 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAK16 fusion protein Ag11705
Full Name MAK16 homolog (S. cerevisiae)
Calculated Molecular Weight 300 aa, 35 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC050528
Gene Symbol MAK16
Gene ID (NCBI) 84549
RRIDAB_2281504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAK16 antibody 17505-1-APDownload protocol
IF protocol for MAK16 antibody 17505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols