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MAK Polyclonal antibody

MAK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13638-1-AP

Synonyms

MAK

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of PC-3 cells using MAK Polyclonal antibody (13638-1-AP)

    WB analysis of PC-3 using 13638-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13638-1-AP (MAK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using MAK Polyclonal antibody (13638-1-AP)

    WB analysis of PC-3 using 13638-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13638-1-AP (MAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MAK Polyclonal antibody (13638-1-AP)

    WB analysis of HeLa using 13638-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13638-1-AP (MAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inPC-3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13638-1-AP targets MAK in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAK fusion protein Ag4571
Full Name male germ cell-associated kinase
Calculated Molecular Weight 623 aa, 71 kDa
Observed Molecular Weight 60-62 kDa
GenBank Accession NumberBC039825
Gene Symbol MAK
Gene ID (NCBI) 4117
RRIDAB_10598167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAK antibody 13638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Biol Chem

Serine/Threonine Kinase Unc-51-like Kinase-1 (Ulk1) Phosphorylates the Co-chaperone Cell Division Cycle Protein 37 (Cdc37) and Thereby Disrupts the Stability of Cdc37 Client Proteins.

Authors - Ran Li
View Article