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KD/KO Validated

MAGED2 Polyclonal antibody

Name: MAGED2 Polyclonal antibody
Brand: Proteintech
SKU: 15252-1-AP

MAGED2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15252-1-AP

Synonyms

JCL 1, Hepatocellular carcinoma-associated protein JCL-1, HCA10, Breast cancer-associated gene 1 protein, BCG-1

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis using 15252-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15252-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 15252-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15252-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 15252-1-AP
IP result of anti-MAGED2 (IP:15252-1-AP, 4ug; Detection:15252-1-AP 1:1000) with HepG2 cells lysate 3200ug.
IHC staining of human breast cancer using 15252-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15252-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 15252-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MAGED2 antibody (15252-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), F-actin was stained using CoraLite®594-phalloidin (red) and DNA was stained by DAPI (blue).

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Cat No. 15252-1-AP

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Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells, A431 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

15252-1-AP targets MAGED2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MAGED2 fusion protein Ag6828
Full Name	melanoma antigen family D, 2
Calculated Molecular Weight	65 kDa
Observed Molecular Weight	65 kDa
GenBank Accession Number	BC000304
Gene Symbol	MAGED2
Gene ID (NCBI)	10916
RRID	AB_2138557
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAGED2 antibody 15252-1-AP	Download protocol
IHC protocol for MAGED2 antibody 15252-1-AP	Download protocol
IF protocol for MAGED2 antibody 15252-1-AP	Download protocol
IP protocol for MAGED2 antibody 15252-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IHC	Nephrol Dial Transplant MAGE genes in the kidney: identification of MAGED2 as upregulated during kidney injury and in stressed tubular cells. Authors - Lara Valiño-Rivas View Article
mouse	WB	J Neurosci MAGE-D1 regulates expression of depression-like behavior through serotonin transporter ubiquitylation. Authors - Mouri Akihiro A View Article
human	WB	Biochem Pharmacol Melanoma antigen-D2 controls cell cycle progression and modulates the DNA damage response. Authors - Charlotte Trussart KD Validated View Article
human	WB	RNA An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21. Authors - Ewan K S McRae View Article
human	WB,IF	Biochim Biophys Acta Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress. Authors - Céline Pirlot KD Validated View Article
human	WB,IP	mBio SARS-CoV-2 main protease cleaves MAGED2 to antagonize host antiviral defense Authors - Xiaohui Ju KD Validated View Article

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MAGED2 Polyclonal antibody

MAGED2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 15252-1-AP

WB analysis of HepG2 using 15252-1-AP

WB analysis of A431 using 15252-1-AP

WB analysis of MCF-7 using 15252-1-AP

IP experiment of HepG2 using 15252-1-AP

IHC staining of human breast cancer using 15252-1-AP

IHC staining of human breast cancer using 15252-1-AP

IF Staining of MCF-7 using 15252-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

MAGE genes in the kidney: identification of MAGED2 as upregulated during kidney injury and in stressed tubular cells.

MAGE-D1 regulates expression of depression-like behavior through serotonin transporter ubiquitylation.

Melanoma antigen-D2 controls cell cycle progression and modulates the DNA damage response.

An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21.

Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress.

SARS-CoV-2 main protease cleaves MAGED2 to antagonize host antiviral defense