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  • Featured Product
  • KD/KO Validated

MAGED1 Polyclonal antibody

MAGED1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 22053-1-AP

Synonyms

PP2250, NRAGE, Neurotrophin receptor-interacting MAGE homolog, Melanoma-associated antigen D1, MAGE-D1 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using MAGED1 Polyclonal antibody (22053-1-AP)

    WB analysis of A549 using 22053-1-AP

    WB result of MAGED1 antibody (22053-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAGED1 transfected A549 cells.

  • Western Blot (WB) analysis of A549 cells using MAGED1 Polyclonal antibody (22053-1-AP)

    WB analysis of A549 using 22053-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAGED1 Polyclonal antibody (22053-1-AP)

    WB analysis using 22053-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MAGED1 Polyclonal antibody (22053-1-AP)

    WB analysis of HEK-293 using 22053-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MAGED1 Polyclonal antibody (22053-1-AP)

    WB analysis of HEK-293 using 22053-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse placenta tissue using MAGED1 Polyclonal antibody (22053-1-AP)

    WB analysis of mouse placenta using 22053-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using MAGED1 Polyclonal antibody (22053-1-AP)

    IP experiment of A549 using 22053-1-AP

    IP result of anti-MAGED1 (IP:22053-1-AP, 4ug; Detection:22053-1-AP 1:1000) with A549 cells lysate 400ug.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using MAGED1 Polyclonal antibody (22053-1-AP)

    IHC staining of mouse placenta using 22053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 22053-1-AP (MAGED1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using MAGED1 Polyclonal antibody (22053-1-AP)

    IHC staining of mouse placenta using 22053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 22053-1-AP (MAGED1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MAGED1 Polyclonal antibody (22053-1-AP)

    IHC staining of human placenta using 22053-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MAGED1 Polyclonal antibody (22053-1-AP)

    IHC staining of human placenta using 22053-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MAGED1 Polyclonal antibody (22053-1-AP)

    IHC staining of human skin using 22053-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MAGED1 Polyclonal antibody (22053-1-AP)

    IHC staining of human skin using 22053-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MAGED1 Polyclonal antibody (22053-1-AP)

    IF Staining of A549 using 22053-1-AP

    Immunofluorescent analysis of A549 cells using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using MAGED1 Polyclonal antibody (22053-1-AP)

    FC experiment of HeLa using 22053-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAGED1 Polyclonal antibody (22053-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, mouse placenta tissue
Positive IP detected inA549 cells
Positive IHC detected inmouse placenta tissue, human skin tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22053-1-AP targets MAGED1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGED1 fusion protein Ag17047
Full Name melanoma antigen family D, 1
Calculated Molecular Weight 834 aa, 92 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC014070
Gene Symbol MAGED1
Gene ID (NCBI) 9500
RRIDAB_11182601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGED1 (also known as NRAGE or Dlxin-1) is a member of the type II melanoma antigen (MAGE) family of protein. MAGED1 can interact with the nerve growth factor receptor p75NTR, antagonize the association of p75NTR with TrkA, inhibit cell cycle progression, and facilitate p75NTR-mediated apoptosis (PMID: 10985348). It has been reported that MAGED1 binds Dlx5 and regulates its transcriptional function. In vivo, MAGED1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homomultimers (PMID: 11084035). MAGED1 knockout mice show depressive behavior and impairments of circadian rhythm (PMID: 20300063; 23314527). Three transcript variants encoding two different isoforms (86kDa and 92 kDa) have been found for the gene of MAGED1.

Protocols

Product Specific Protocols
WB protocol for MAGED1 antibody 22053-1-APDownload protocol
IHC protocol for MAGED1 antibody 22053-1-APDownload protocol
IF protocol for MAGED1 antibody 22053-1-APDownload protocol
IP protocol for MAGED1 antibody 22053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Clin Transl Med

Saikosaponin-d impedes hippocampal neurogenesis and causes cognitive deficits by inhibiting the survival of neural stem/progenitor cells via neurotrophin receptor signaling in mice.

Authors - Tingting Qin
View Article
mouseWB

Biochim Biophys Acta Mol Cell Biol Lipids

Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system.

Authors - Qiang Lu
View Article
humanWB,IHC,IF

Tumour Biol

Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells.

Authors - Huandi Zhou
  • KD Validated
View Article
humanWB,IHC

Gene

NRAGE confers poor prognosis and promotes proliferation, invasion, and chemoresistance in gastric cancer.

Authors - Xiaodi Jiang
  • KD Validated
View Article
humanWB

bioRxiv

Histone chaperone HIRA, Promyelocytic Leukemia (PML) protein and p62/SQSTM1 coordinate to regulate inflammation during cell senescence

Authors - Nirmalya Dasgupta
View Article