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MAGEC2 Polyclonal antibody

MAGEC2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 10280-1-AP

Synonyms

Cancer/testis antigen 10, CT10, HCA587, MAGE C2 antigen, MAGE E1 antigen, MAGEC2, MAGEE1, melanoma antigen family C, 2, Melanoma associated antigen C2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MAGEC2 Polyclonal antibody (10280-1-AP)

    WB analysis of HEK-293 using 10280-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10280-1-AP (MAGEC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MAGEC2 Polyclonal antibody (10280-1-AP)

    IHC staining of human liver cancer using 10280-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10280-1-AP (MAGEC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MAGEC2 Polyclonal antibody (10280-1-AP)

    IHC staining of human liver cancer using 10280-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10280-1-AP (MAGEC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using MAGEC2 Polyclonal antibody (10280-1-AP)

    IHC staining of human testis using 10280-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10280-1-AP (MAGEC2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MAGEC2 Polyclonal antibody (10280-1-AP)

    IHC staining of human testis using 10280-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10280-1-AP (MAGEC2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using MAGEC2 Polyclonal antibody (10280-1-AP)

    IHC staining of human malignant melanoma using 10280-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10280-1-AP (MAGEC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using MAGEC2 Polyclonal antibody (10280-1-AP)

    IHC staining of human malignant melanoma using 10280-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10280-1-AP (MAGEC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAGEC2 Polyclonal antibody (10280-1-AP)

    IHC staining of human breast cancer using 10280-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10280-1-AP (MAGEC2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAGEC2 Polyclonal antibody (10280-1-AP)

    IHC staining of human breast cancer using 10280-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10280-1-AP (MAGEC2 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human malignant melanoma tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

10280-1-AP targets MAGEC2 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEC2 fusion protein Ag0418
Full Name melanoma antigen family C, 2
Calculated Molecular Weight 373 aa, 41 kDa
GenBank Accession NumberBC005891
Gene Symbol MAGEC2
Gene ID (NCBI) 51438
RRIDAB_2138540
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Melanoma antigen family C, 2(MAGEC2, synonyms: CT10, HCA587, MAGEE1, MGC13377) is related to members of the MAGEC gene family. MAGEC2 is not expressed in normal tissues, except for testis, and is expressed in tumors of various histological types. This gene and other MAGEC genes are clustered on chromosome Xq26-q27.

Protocols

Product Specific Protocols
WB protocol for MAGEC2 antibody 10280-1-APDownload protocol
IHC protocol for MAGEC2 antibody 10280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

PLoS One

γ-Radiation promotes immunological recognition of cancer cells through increased expression of cancer-testis antigens in vitro and in vivo.

Authors - Sharma Anu A
View Article
humanIHC

Hum Pathol

Primary mediastinal seminomas: a comprehensive immunohistochemical study with a focus on novel markers.

Authors - Annikka Weissferdt
View Article

Laryngoscope Investig Otolaryngol

The expression of the cancer testis antigen MAGE A4: A favorable prognostic biomarker in salivary gland carcinomas related to low tumor grading.

Authors - Domenic Vital
View Article