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MAGEB4 Polyclonal antibody

MAGEB4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12786-2-AP

Synonyms

Melanoma-associated antigen B4, MAGE-B4 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGEB4 Polyclonal antibody (12786-2-AP)

    WB analysis using 12786-2-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12786-2-AP (MAGEB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MAGEB4 Polyclonal antibody (12786-2-AP)

    WB analysis of K-562 using 12786-2-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12786-2-AP (MAGEB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using MAGEB4 Polyclonal antibody (12786-2-AP)

    WB analysis of human testis using 12786-2-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12786-2-AP (MAGEB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using MAGEB4 Polyclonal antibody (12786-2-AP)

    IP experiment of K-562 using 12786-2-AP

    IP result of anti-MAGEB4 (IP:12786-2-AP, 4ug; Detection:12786-2-AP 1:300) with K-562 cells lysate 4000 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAGEB4 Polyclonal antibody (12786-2-AP)

    IF Staining of HepG2 using 12786-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MAGEB4 antibody (12786-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue, K-562 cells
Positive IP detected inK-562 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12786-2-AP targets MAGEB4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEB4 fusion protein Ag3502
Full Name melanoma antigen family B, 4
Calculated Molecular Weight 346 aa, 39 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC032852
Gene Symbol MAGEB4
Gene ID (NCBI) 4115
RRIDAB_2138418
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAGEB4 antibody 12786-2-APDownload protocol
IF protocol for MAGEB4 antibody 12786-2-APDownload protocol
IP protocol for MAGEB4 antibody 12786-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols