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MAGEB18 Polyclonal antibody

MAGEB18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17494-1-AP

Synonyms

MAGE B18 antigen, MAGEB18, melanoma antigen family B, 18

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGEB18 Polyclonal antibody (17494-1-AP)

    WB analysis using 17494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MAGEB18 Polyclonal antibody (17494-1-AP)

    WB analysis of HeLa using 17494-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using MAGEB18 Polyclonal antibody (17494-1-AP)

    WB analysis of A549 using 17494-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MAGEB18 Polyclonal antibody (17494-1-AP)

    WB analysis of MCF-7 using 17494-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human testis using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human testis using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human brain using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human brain using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human spleen using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human spleen using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human lung using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human lung using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human ovary using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human ovary using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human heart using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human heart using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human kidney using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human kidney using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human placenta using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MAGEB18 Polyclonal antibody (17494-1-AP)

    IHC staining of human placenta using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MAGEB18 Polyclonal antibody (17494-1-AP)

    IF Staining of A549 using 17494-1-AP

    Immunofluorescent analysis of A549 cells using 17494-1-AP (MAGEB18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA375 cells, HeLa cells, A549 cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17494-1-AP targets MAGEB18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEB18 fusion protein Ag11624
Full Name melanoma antigen family B, 18
Calculated Molecular Weight 343 aa, 39 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC029525
Gene Symbol MAGEB18
Gene ID (NCBI) 286514
RRIDAB_2138404
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAGEB18 antibody 17494-1-APDownload protocol
IHC protocol for MAGEB18 antibody 17494-1-APDownload protocol
IF protocol for MAGEB18 antibody 17494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols