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MAGEA8 Polyclonal antibody

MAGEA8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15045-1-AP

Synonyms

Melanoma-associated antigen 8, MAGE-8 antigen, MAGE8, CT1.8, Cancer/testis antigen 1.8

Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using MAGEA8 Polyclonal antibody (15045-1-AP)

    WB analysis of A375 using 15045-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15045-1-AP (MAGEA8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using MAGEA8 Polyclonal antibody (15045-1-AP)

    IHC staining of human testis using 15045-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15045-1-AP (MAGEA8 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MAGEA8 Polyclonal antibody (15045-1-AP)

    IHC staining of human testis using 15045-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15045-1-AP (MAGEA8 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MAGEA8 Polyclonal antibody (15045-1-AP)

    IF Staining of A375 using 15045-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 15045-1-AP (MAGEA8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA375 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15045-1-AP targets MAGEA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEA8 fusion protein Ag6984
Full Name melanoma antigen family A, 8
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC002455
Gene Symbol MAGEA8
Gene ID (NCBI) 4107
RRIDAB_2138289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAGEA8 antibody 15045-1-APDownload protocol
IHC protocol for MAGEA8 antibody 15045-1-APDownload protocol
IF protocol for MAGEA8 antibody 15045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols