MAGEA4 Polyclonal antibody
MAGEA4 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 12508-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, U2OS cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IHC | See 1 publications below |
Product Information
12508-1-AP targets MAGEA4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MAGEA4 fusion protein Ag3193 |
Full Name | melanoma antigen family A, 4 |
Calculated Molecular Weight | 317 aa, 35 kDa |
Observed Molecular Weight | 45 kDa |
GenBank Accession Number | BC017723 |
Gene Symbol | MAGEA4 |
Gene ID (NCBI) | 4103 |
RRID | AB_2877863 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for MAGEA4 antibody 12508-1-AP | Download protocol |
IHC protocol for MAGEA4 antibody 12508-1-AP | Download protocol |
FC protocol for MAGEA4 antibody 12508-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Endocrinol (Lausanne) Four novel mutations identification in 17 beta-hydroxysteroid dehydrogenase-3 deficiency and our clinical experience: possible benefits of early treatment |