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MAGEA3 Monoclonal antibody

MAGEA3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A9F3

Cat no : 60054-1-Ig

Synonyms

Antigen MZ2 D, Cancer/testis antigen 1.3, CT1.3, HIP8, HYPD, MAGE 3 antigen, MAGE3, MAGEA3, MAGE-A3, MAGEA6, melanoma antigen family A, 3, Melanoma associated antigen 3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    WB analysis of mouse brain using 60054-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    WB analysis of rat brain using 60054-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    WB analysis of human testis using 60054-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    WB analysis of fetal human brain using 60054-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    WB analysis of rat brain using 60054-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    WB analysis of mouse brain using 60054-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human lung cancer using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human lung cancer using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human oesophagus cancer using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human oesophagus cancer using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human lung cancer using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human lung cancer using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human lung cancer using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human lung cancer using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human lung cancer using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:25 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human lung cancer using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:25 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human testis using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human testis using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human lung cancer using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAGEA3 Monoclonal antibody (60054-1-Ig)

    IHC staining of human lung cancer using 60054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue, human testis tissue, rat brain tissue
Positive IHC detected inhuman lung cancer tissue, human oesophagus cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

60054-1-Ig targets MAGEA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAGEA3 fusion protein Ag0265
Full Name melanoma antigen family A, 3
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight45-48 kDa
GenBank Accession NumberBC000340
Gene Symbol MAGEA3
Gene ID (NCBI) 4102
RRIDAB_2138124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGEA3 is a member of the MAGEA family, , which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.

Protocols

Product Specific Protocols
WB protocol for MAGEA3 antibody 60054-1-IgDownload protocol
IHC protocol for MAGEA3 antibody 60054-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

MAGE-A3 is a prognostic biomarker for poor clinical outcome in cutaneous squamous cell carcinoma with perineural invasion via modulation of cell proliferation.

Authors - Aaron Chen
View Article
humanWB

J Transl Med

Immunization with a multi-antigen targeted DNA vaccine eliminates chemoresistant pancreatic cancer by disrupting tumor-stromal cell crosstalk

Authors - Hongquan Qin
View Article