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MAGEA1 Polyclonal antibody

MAGEA1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55105-1-AP

Synonyms

Antigen MZ2 E, Cancer/testis antigen 1.1, CT1.1, MAGE 1 antigen, MAGE1, MAGE1A, MAGEA1, Melanoma associated antigen 1

Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using MAGEA1 Polyclonal antibody (55105-1-AP)

    WB analysis of A375 using 55105-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 55105-1-AP (MAGEA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MAGEA1 Polyclonal antibody (55105-1-AP)

    WB analysis of MCF-7 using 55105-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 55105-1-AP (MAGEA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MAGEA1 Polyclonal antibody (55105-1-AP)

    IF Staining of A375 using 55105-1-AP

    Immunofluorescent analysis of A375 cells using 55105-1-AP (MAGEA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA375 cells, MCF-7 cells
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55105-1-AP targets MAGEA1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberNM_004988
Gene Symbol MAGEA1
Gene ID (NCBI) 4100
RRIDAB_10859942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGEA1, also named as CT1.1, MZ2-E, MAGE1 and MAGE1A, is the first gene reported to encode a CTL-defined human tumor antigen. It may play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. MAGEA1 provided direct evidence for the power of the cDNA expression library as an effective strategy to identify genes encoding tumor antigens recognized by HLA-restricted T cells.(PMID:16951217 ) The antibody is specific to MAGEA1.

Protocols

Product Specific Protocols
WB protocol for MAGEA1 antibody 55105-1-APDownload protocol
IF protocol for MAGEA1 antibody 55105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols