MAG Polyclonal antibody
MAG Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 14386-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse spinal cord tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 16 publications below |
| IF | See 5 publications below |
Product Information
14386-1-AP targets MAG in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MAG fusion protein Ag5845 |
| Full Name | myelin associated glycoprotein |
| Calculated Molecular Weight | 69 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC053347 |
| Gene Symbol | MAG |
| Gene ID (NCBI) | 4099 |
| RRID | AB_2878051 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAG antibody 14386-1-AP | Download protocol |
| IHC protocol for MAG antibody 14386-1-AP | Download protocol |
| IF protocol for MAG antibody 14386-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain A role of the frontotemporal lobar degeneration risk factor TMEM106B in myelination. | ||
Acta Neuropathol Commun TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss. | ||
J Cereb Blood Flow Metab Connexin43 promotes angiogenesis through activating the HIF-1α/VEGF signaling pathway under chronic cerebral hypoperfusion. | ||
Mol Neurobiol Quercetin Alleviates Demyelination Through Regulating Microglial Phenotype Transformation to Mitigate Neuropsychiatric Symptoms in Mice with Vascular Dementia. | ||
Neurosci Bull Digoxin Ameliorates Glymphatic Transport and Cognitive Impairment in a Mouse Model of Chronic Cerebral Hypoperfusion | ||
CNS Neurosci Ther Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic-ischemic white matter damage. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Emma (Verified Customer) (02-22-2022) | Works well on FFPE prostate tissue by IF. (Tris-EDTA antigen retrieval).
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