• Featured Product
  • KD/KO Validated

MAFF Polyclonal antibody

MAFF Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12771-1-AP

Synonyms

hMafF, MAFF, Transcription factor MafF, U MAF



Tested Applications

Positive WB detected inhuman lung tissue
Positive IP detected inSKOV-3 cells
Positive IHC detected inhuman ovary tumor tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12771-1-AP targets MAFF in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAFF fusion protein Ag3460
Full Name v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)
Calculated Molecular Weight 17 aa, 8 kDa
Observed Molecular Weight 18-23 kDa
GenBank Accession NumberBC015037
Gene Symbol MAFF
Gene ID (NCBI) 23764
RRIDAB_2137677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889).

Protocols

Product Specific Protocols
WB protocol for MAFF antibody 12771-1-APDownload protocol
IHC protocol for MAFF antibody 12771-1-APDownload protocol
IP protocol for MAFF antibody 12771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Sci Transl Med

PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.

Authors - Tian-Yi Jiang
human,mouseWB,IHC,IF

J Clin Invest

circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.

Authors - Zhenzhen Chen
humanWB

Autophagy

The FACT complex facilitates expression of lysosomal and antioxidant genes through binding to TFEB and TFE3.

Authors - Eutteum Jeong
mouse,ratWB

Mol Ther Nucleic Acids

miR-320a induces pancreatic β cells dysfunction in diabetes by inhibiting MafF

Authors - Hengzhi Du
mouseIF

BMC Genomics

Targeted insertion of conditional expression cassettes into the mouse genome using the modified i-PITT

Authors - Hiromi Miura
humanWB

Mol Ther Oncolytics

RBFOX1 Regulates the Permeability of the Blood-Tumor Barrier via the LINC00673/MAFF Pathway.

Authors - Shuyuan Shen
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Amy (Verified Customer) (03-18-2024)

Relatively clean bands but not very strong, U2OS lysates 25ug.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: U2OS