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  • Featured Product
  • KD/KO Validated

MAEL Polyclonal antibody

MAEL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, RIP, ELISA

Conjugate

Unconjugated

Cat no : 26666-1-AP

Synonyms

MAEL, maelstrom homolog (Drosophila), Protein maelstrom homolog, RP11 102C16.1

Validation Data Gallery

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  • Western Blot (WB) analysis of rat testis tissue using MAEL Polyclonal antibody (26666-1-AP)

    WB analysis of rat testis using 26666-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 26666-1-AP (MAEL Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using MAEL Polyclonal antibody (26666-1-AP)

    WB analysis of mouse testis using 26666-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26666-1-AP (MAEL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using MAEL Polyclonal antibody (26666-1-AP)

    IHC staining of human testis using 26666-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26666-1-AP (MAEL Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MAEL Polyclonal antibody (26666-1-AP)

    IHC staining of human testis using 26666-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26666-1-AP (MAEL Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MAEL Polyclonal antibody (26666-1-AP)

    IHC staining of mouse testis using 26666-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26666-1-AP (MAEL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inrat testis tissue, mouse testis tissue
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26666-1-AP targets MAEL in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAEL fusion protein Ag24524
Full Name maelstrom homolog (Drosophila)
Calculated Molecular Weight 434 aa, 49 kDa
Observed Molecular Weight 46-50 kDa
GenBank Accession NumberBC028595
Gene Symbol MAEL
Gene ID (NCBI) 84944
RRIDAB_2880595
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAEL antibody 26666-1-APDownload protocol
IHC protocol for MAEL antibody 26666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,RIP

Andrology

The MAEL expression in mitochondria of human spermatozoa and the association with asthenozoospermia

Authors - Yu-Sheng Cheng
  • KD Validated
View Article
mouseWB

J Cell Mol Med

Loss-of-function variant in TDRD6 cause male infertility with severe oligo-astheno-teratozoospermia in human and mice

Authors - Xinying Bi
View Article