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MAEA Polyclonal antibody

MAEA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15238-1-AP

Synonyms

EMLP, EMP, HLC 10, MAEA, PIG5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MAEA Polyclonal antibody (15238-1-AP)

    WB analysis using 15238-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15238-1-AP (MAEA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAEA Polyclonal antibody (15238-1-AP)

    IHC staining of human lung cancer using 15238-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15238-1-AP (MAEA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAEA Polyclonal antibody (15238-1-AP)

    IHC staining of human lung cancer using 15238-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15238-1-AP (MAEA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MAEA Polyclonal antibody (15238-1-AP)

    IHC staining of human lung cancer using 15238-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15238-1-AP (MAEA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAEA Polyclonal antibody (15238-1-AP)

    IHC staining of human breast cancer using 15238-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15238-1-AP (MAEA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAEA Polyclonal antibody (15238-1-AP)

    IHC staining of human breast cancer using 15238-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15238-1-AP (MAEA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells, U2OS cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15238-1-AP targets MAEA in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAEA fusion protein Ag7365
Full Name macrophage erythroblast attacher
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC001225
Gene Symbol MAEA
Gene ID (NCBI) 10296
RRIDAB_2137505
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).

Protocols

Product Specific Protocols
WB protocol for MAEA antibody 15238-1-APDownload protocol
IHC protocol for MAEA antibody 15238-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24

Authors - Lalit R Patel
View Article