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MAdCAM1 Polyclonal antibody

MAdCAM1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21917-1-AP

Synonyms

hMAdCAM 1, MACAM1, MAdCAM1

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human small intestine tissue using MAdCAM1 Polyclonal antibody (21917-1-AP)

    IHC staining of human small intestine using 21917-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21917-1-AP (MAdCAM1 antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using MAdCAM1 Polyclonal antibody (21917-1-AP)

    IHC staining of human small intestine using 21917-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21917-1-AP (MAdCAM1 antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using MAdCAM1 Polyclonal antibody (21917-1-AP)

    IHC staining of human small intestine using 21917-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21917-1-AP (MADCAM1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using MAdCAM1 Polyclonal antibody (21917-1-AP)

    IHC staining of human small intestine using 21917-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21917-1-AP (MADCAM1 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21917-1-AP is MAdCAM1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAdCAM1 fusion protein Ag16184
Full Name mucosal vascular addressin cell adhesion molecule 1
Calculated Molecular Weight 406 aa, 43 kDa
GenBank Accession NumberBC142629
Gene Symbol MADCAM1
Gene ID (NCBI) 8174
RRIDAB_2802114
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.

Protocols

Product Specific Protocols
IHC protocol for MAdCAM1 antibody 21917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Immunity

T Cell Recruitment to the Intestinal Stem Cell Compartment Drives Immune-Mediated Intestinal Damage after Allogeneic Transplantation.

Authors - Ya-Yuan Fu
View Article
WB

Oncotarget

Cytological study on the regulation of lymphocyte homing in the chicken spleen during LPS stimulation.

Authors - Qian Zhang
View Article
humanWB,IHC

Cell Death Dis

Integrated DNA and RNA sequencing reveals early drivers involved in metastasis of gastric cancer.

Authors - Jieyun Zhang
View Article