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  • Featured Product
  • KD/KO Validated

MAD2L1 Polyclonal antibody

MAD2L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10337-1-AP

Synonyms

Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2 like protein 1, MAD2, HSMAD2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAD2L1 Polyclonal antibody (10337-1-AP)

    WB analysis using 10337-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAD2L1 Polyclonal antibody (10337-1-AP)

    WB analysis using 10337-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MAD2L1 Polyclonal antibody (10337-1-AP)

    WB analysis of HEK-293 using 10337-1-AP

    WB result of MAD2L1 antibody (10337-1-AP, 1:500) with si-control and si-MAD2L1 transfected HEK293 cells.

  • Western Blot (WB) analysis of rat liver tissue using MAD2L1 Polyclonal antibody (10337-1-AP)

    WB analysis of rat liver using 10337-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MAD2L1 Polyclonal antibody (10337-1-AP)

    IP experiment of HEK-293 using 10337-1-AP

    IP result of anti-MAD2L1 (IP:10337-1-AP, 3ug; Detection:10337-1-AP 1:500) with HEK-293 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MAD2L1 Polyclonal antibody (10337-1-AP)

    IHC staining of human liver cancer using 10337-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 25x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using MAD2L1 Polyclonal antibody (10337-1-AP)

    IHC staining of human ovary tumor using 10337-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MAD2L1 Polyclonal antibody (10337-1-AP)

    IHC staining of human liver cancer using 10337-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MAD2L1 Polyclonal antibody (10337-1-AP)

    IHC staining of human liver cancer using 10337-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using MAD2L1 Polyclonal antibody (10337-1-AP)

    IHC staining of human ovary tumor using 10337-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using MAD2L1 Polyclonal antibody (10337-1-AP)

    IHC staining of human ovary tumor using 10337-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAD2L1 Polyclonal antibody (10337-1-AP)

    IF Staining of HepG2 using 10337-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAD2L1 antibody (10337-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MAD2L1 Polyclonal antibody (10337-1-AP)

    IF Staining of HEK-293 using 10337-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inDaudi cells, rat liver tissue, HEK-293 cells, HepG2 cells, Raji cells, THP-1 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10337-1-AP targets MAD2L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAD2L1 fusion protein Ag0307
Full Name MAD2 mitotic arrest deficient-like 1 (yeast)
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC000356
Gene Symbol MAD2L1
Gene ID (NCBI) 4085
RRIDAB_2139365
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.

Protocols

Product Specific Protocols
WB protocol for MAD2L1 antibody 10337-1-APDownload protocol
IHC protocol for MAD2L1 antibody 10337-1-APDownload protocol
IF protocol for MAD2L1 antibody 10337-1-APDownload protocol
IP protocol for MAD2L1 antibody 10337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Sci Adv

The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I.

Authors - Changyin Zhou
View Article
humanWB,IF

Nucleic Acids Res

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Authors - Fengjie Yuan
View Article
IF

J Cell Biol

Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I.

Authors - Tianyu Wu
View Article
humanWB

Oncogene

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Authors - Zhi-Chuan Zhu
View Article
WB,IHC

Cancer Res

Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis.

Authors - He Feng F
View Article

Cancer Res

MPHOSPH1: a potential therapeutic target for hepatocellular carcinoma.

Authors - Xinran Liu
View Article