MAD1 Polyclonal antibody
MAD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 18322-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, human brain tissue, Raji cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 1 publications below |
Product Information
18322-1-AP targets MAD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MAD1 fusion protein Ag13171 |
| Full Name | MAD1 mitotic arrest deficient-like 1 (yeast) |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC009964 |
| Gene Symbol | MAD1 |
| Gene ID (NCBI) | 8379 |
| RRID | AB_2139251 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAD1L1 is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. It recruits MAD2L1 to unattached kinetochores. Also it is required for anchoring MAD2L1 to the nuclear periphery. In addition, it binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding [PMID:10049595, 12837246].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAD1 antibody 18322-1-AP | Download protocol |
| IHC protocol for MAD1 antibody 18322-1-AP | Download protocol |
| IF protocol for MAD1 antibody 18322-1-AP | Download protocol |
| IP protocol for MAD1 antibody 18322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Cycle Nuclear interactor of ARF and Mdm2 regulates multiple pathways to activate p53. | ||
BMB Rep TCP10L synergizes with MAD1 in transcriptional suppression and cell cycle arrest through mutual interaction. | ||
Sci Adv Biallelic germline mutations in MAD1L1 induce a syndrome of aneuploidy with high tumor susceptibility | ||
Aging (Albany NY) The HNF4A-CHPF pathway promotes proliferation and invasion through interactions with MAD1L1 in glioma |
