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- KD/KO Validated
LZTFL1 Polyclonal antibody
LZTFL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 17073-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, U-251 cells, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human colon tissue, human heart tissue, human lung tissue, human small intestine tissue, human testis tissue, mouse heart tissue, mouse lung tissue, mouse small intestine tissue, mouse testis tissue, rat heart tissue, rat small intestine tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
17073-1-AP targets LZTFL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LZTFL1 fusion protein Ag10919 |
| Full Name | leucine zipper transcription factor-like 1 |
| Calculated Molecular Weight | 299 aa, 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC025988 |
| Gene Symbol | LZTFL1 |
| Gene ID (NCBI) | 54585 |
| RRID | AB_10950400 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LZTFL1 antibody 17073-1-AP | Download protocol |
| IHC protocol for LZTFL1 antibody 17073-1-AP | Download protocol |
| IF protocol for LZTFL1 antibody 17073-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.
| ||
EMBO J Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling. | ||
J Cell Biol Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling. | ||
Oncogene LZTFL1 inhibits kidney tumor cell growth by destabilizing AKT through ZNRF1-mediated ubiquitin proteosome pathway | ||
Mol Immunol Anti-inflammation of LZTFL1 knockdown in OVA-induced asthmatic mice: Through ERK/GATA3 signaling pathway |
