LYPLA1 Polyclonal antibody

LYPLA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16055-1-AP

Synonyms

EC:3.1.2.22, EC:3.1.2.-, APT-1, APT1, APT 1



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, human brain tissue, human liver tissue, rat liver tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16055-1-AP targets LYPLA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LYPLA1 fusion protein Ag9022
Full Name lysophospholipase I
Calculated Molecular Weight 230 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC010397
Gene Symbol LYPLA1
Gene ID (NCBI) 10434
RRIDAB_2234851
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LYPLA1(Lysophospholipase 1) is also named as APT1(Acyl-protein thioesterase 1), LPL1 and belongs to the AB hydrolase 2 family. It is an important enzyme responsible for depalmitoylation of palmitoyl proteins and works as a thioesterase that cleaves the S-palmitoyl group of H-Ras, heterotrimeric G protein alfa subunit (Ga) , regulator of G protein signaling 4 (RGS4) and the endothelial isoform of nitric oxide synthase (eNOS) in vitro(PMID:19439193).

Protocols

Product Specific Protocols
WB protocol for LYPLA1 antibody 16055-1-APDownload protocol
IHC protocol for LYPLA1 antibody 16055-1-APDownload protocol
IF protocol for LYPLA1 antibody 16055-1-APDownload protocol
IP protocol for LYPLA1 antibody 16055-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Transl Med

Identifying prognostic markers in spatially heterogeneous breast cancer microenvironment

Authors - Guohong Liu
humanWB

Ecotoxicol Environ Saf

PM2.5 promoted lipid accumulation in macrophage via inhibiting JAK2/STAT3 signaling pathways and aggravating the inflammatory reaction.

Authors - Liwei Yang
humanWB

PLoS One

Development of an activity-based probe for acyl-protein thioesterases.

Authors - Megan Garland
humanIHC

Drug Metab Dispos

Identification of Bioactivating Enzymes Involved in the Hydrolysis of Laninamivir Octanoate, a Long-acting Neuraminidase Inhibitor, in Human Pulmonary Tissue.

Authors - Kumiko Koyama
humanWB

Autophagy

Hypoxia-induced GPCPD1 depalmitoylation triggers mitophagy via regulating PRKN-mediated ubiquitination of VDAC1

Authors - Ying Liu
humanIHC,WB

Reprod Sci

Estrogen Regulates Scribble Localization in Endometrial Epithelial Cells Through Acyl Protein Thioesterase (APT)-Mediated S-Palmitoylation in Adenomyosis

Authors - Zhixing Jin