LYN Polyclonal antibody
LYN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 18135-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HL-60 cells, Jurkat cells, K-562 cells, L02 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 18135-1-AP is LYN Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LYN fusion protein Ag12723 |
| Full Name | v-yes-1 Yamaguchi sarcoma viral related oncogene homolog |
| Calculated Molecular Weight | 512 aa, 59 kDa |
| Observed Molecular Weight | 53-56 kDa, 66-70 kDa |
| GenBank Accession Number | BC075002 |
| Gene Symbol | LYN |
| Gene ID (NCBI) | 4067 |
| RRID | AB_11042319 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LYN antibody 18135-1-AP | Download protocol |
| IHC protocol for LYN antibody 18135-1-AP | Download protocol |
| IF protocol for LYN antibody 18135-1-AP | Download protocol |
| IP protocol for LYN antibody 18135-1-AP | Download protocol |
| FC protocol for LYN antibody 18135-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Invest Dermatol Loss of LYN is frequent in targeted-therapy resistant melanoma cells and favors metastatic properties
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