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Lumican Polyclonal antibody

Lumican Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 10677-1-AP

Synonyms

LUM, SLRR2D, LDC, KSPG lumican

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Lumican Polyclonal antibody (10677-1-AP)

    WB analysis of Jurkat using 10677-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10677-1-AP (Lumican antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Lumican Polyclonal antibody (10677-1-AP)

    WB analysis of Jurkat using 10677-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10677-1-AP (Lumican antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Lumican Polyclonal antibody (10677-1-AP)

    WB analysis of Jurkat using 10677-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10677-1-AP (Lumican antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using Lumican Polyclonal antibody (10677-1-AP)

    IF Staining of human colon using 10677-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Lumican antibody (10677-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells
Positive IF-P detected inhuman colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

10677-1-AP targets Lumican in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Lumican fusion protein Ag1106
Full Name lumican
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 65-90 kDa
GenBank Accession NumberBC007038
Gene Symbol Lumican
Gene ID (NCBI) 4060
RRIDAB_2139481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lumican (LUM), a member of the small leucine-rich proteoglycan (SLRP) family, is one of the major extracellular components in interstitial collagenous matrices of the corneal stroma, aorta, skin, skeletal muscle, lung, kidney, bone, cartilage, and intervertebral discs. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins and have important effects on cell behavior. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and may exist as a glycoprotein in the connective tissues of other organs. Posttranslational modifications lumican goes through can increase its apparent molecular weight to 50-90 kDa. Studies show that lumican participates in the maintenance of tissue homeostasis and modulates cellular functions including cell proliferation, migration, and differentiation. (PMID: 18949819; 22252641; 9761754; 22365843)

Protocols

Product Specific Protocols
WB protocol for Lumican antibody 10677-1-APDownload protocol
IF protocol for Lumican antibody 10677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB

Front Mol Biosci

Integrated Strategies of Diverse Feature Selection Methods Identify Aging-Based Reliable Gene Signatures for Ischemic Cardiomyopathy.

Authors - Huafeng Song
View Article
humanWB

J Proteomics

Protein expression profiles of human lymph and plasma mapped by 2D-DIGE and 1D SDS-PAGE coupled with nanoLC-ESI-MS/MS bottom-up proteomics.

Authors - Clement Cristina C CC
View Article
humanWB

J Cell Physiol

Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.

Authors - He Wang
View Article