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LUC7L2 Polyclonal antibody

LUC7L2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24202-1-AP

Synonyms

LUC7B2, LUC7 like 2 (S. cerevisiae), CGI-74, CGI-59, CGI 74

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using LUC7L2 Polyclonal antibody (24202-1-AP)

    WB analysis of K-562 using 24202-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24202-1-AP (LUC7L2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LUC7L2 Polyclonal antibody (24202-1-AP)

    WB analysis of HeLa using 24202-1-AP

    WB result of LUC7L2 antibody (24202-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LUC7L2 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using LUC7L2 Polyclonal antibody (24202-1-AP)

    WB analysis of HeLa using 24202-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24202-1-AP (LUC7L2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using LUC7L2 Polyclonal antibody (24202-1-AP)

    IF Staining of A431 using 24202-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using LUC7L2 antibody (24202-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inK-562 cells, HeLa cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24202-1-AP targets LUC7L2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LUC7L2 fusion protein Ag21135
Full Name LUC7-like 2 (S. cerevisiae)
Calculated Molecular Weight 392 aa, 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC017163
Gene Symbol LUC7L2
Gene ID (NCBI) 51631
RRIDAB_2879455
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding protein LUC7L2 as a negative regulator of DNA virus-triggered innate immune response. Mechanistically, LUC7L2 directly bound to intron 3 of MITA precursor messenger RNA, inhibited its splicing, and promoted its nonsense-mediated decay, leading to its downregulation at the protein level (PMID:34155193). Moreover, LUC7L2 as a component of the U1 snRNP capable of reprogramming cellular metabolism through changes in alternative pre-mRNA splicing (PMID:33961773).

Protocols

Product Specific Protocols
WB protocol for LUC7L2 antibody 24202-1-APDownload protocol
IF protocol for LUC7L2 antibody 24202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols