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LTA4H Polyclonal antibody

LTA4H Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13662-1-AP

Synonyms

Leukotriene A 4 hydrolase, Leukotriene A(4) hydrolase, leukotriene A4 hydrolase, LTA 4 hydrolase, LTA4, LTA4H

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using LTA4H Polyclonal antibody (13662-1-AP)

    WB analysis of HEK-293 using 13662-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LTA4H Polyclonal antibody (13662-1-AP)

    WB analysis of Jurkat using 13662-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using LTA4H Polyclonal antibody (13662-1-AP)

    WB analysis of HEK-293 using 13662-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LTA4H Polyclonal antibody (13662-1-AP)

    WB analysis of Jurkat using 13662-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using LTA4H Polyclonal antibody (13662-1-AP)

    IP experiment of HEK-293 using 13662-1-AP

    IP result of anti-LTA4H (IP:13662-1-AP, 4ug; Detection:13662-1-AP 1:500) with HEK-293 cells lysate 1120ug.

  • Immunohistochemistry (IHC) staining of human skin tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human skin using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human skin using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human brain using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human brain using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human spleen using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human spleen using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human lung using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human lung using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human ovary using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human ovary using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human heart using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human heart using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human kidney using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human kidney using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human placenta using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human placenta using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human testis using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LTA4H Polyclonal antibody (13662-1-AP)

    IHC staining of human testis using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman skin tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13662-1-AP targets LTA4H in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LTA4H fusion protein Ag4568
Full Name leukotriene A4 hydrolase
Calculated Molecular Weight 611 aa, 69 kDa
Observed Molecular Weight 57-70 kDa
GenBank Accession NumberBC032528
Gene Symbol LTA4H
Gene ID (NCBI) 4048
RRIDAB_2138747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LTA4H(Leukotriene A-4 hydrolase), also named as LTA4, is essential for the conversion of LTA4 to LTB4, an inflammatory lipid mediator(PMID:10601658). LTA4H also possesses aminopeptidase activity that it can degrade PGP, which is a biomarker for chronic obstructive pulmonary disease and is implicated in neutrophil persistence in the lung(PMID:20813919). This protein has 4 isoforms produced by alternative splicing with the MW of 69, 59, 57, 67 kDa.

Protocols

Product Specific Protocols
WB protocol for LTA4H antibody 13662-1-APDownload protocol
IHC protocol for LTA4H antibody 13662-1-APDownload protocol
IP protocol for LTA4H antibody 13662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Signal

ALOX5 drives the pyroptosis of CD4+ T cells and tissue inflammation in rheumatoid arthritis

Authors - Hao Cai
View Article
humanWB

Proteomics

Mass Spectrometric Analysis Identifies AIMP1 and LTA4H as FSCN1-Binding Proteins in Laryngeal Squamous Cell Carcinoma.

Authors - Wei Gao
View Article
mouseIF

JHEP Rep

Time-resolved single-cell transcriptomics reveals the landscape and dynamics of hepatic cells in sepsis-induced acute liver dysfunction

Authors - Gan Chen
View Article