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LSM8 Polyclonal antibody

LSM8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10134-1-AP

Synonyms

YJR022W, U6 snRNA-associated Sm-like protein LSm8, NAA38

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LSM8 Polyclonal antibody (10134-1-AP)

    WB analysis of HeLa using 10134-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10134-1-AP (LSM8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using LSM8 Polyclonal antibody (10134-1-AP)

    WB analysis of HL-60 using 10134-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10134-1-AP (LSM8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LSM8 Polyclonal antibody (10134-1-AP)

    IHC staining of human kidney using 10134-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10134-1-AP (LSM8 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LSM8 Polyclonal antibody (10134-1-AP)

    IHC staining of human kidney using 10134-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10134-1-AP (LSM8 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LSM8 Polyclonal antibody (10134-1-AP)

    IF Staining of HeLa using 10134-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LSM8 antibody (10134-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HL-60 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10134-1-AP targets LSM8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSM8 fusion protein Ag0178
Full Name LSM8 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Calculated Molecular Weight 14 kDa /75 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC002742
Gene Symbol LSM8
Gene ID (NCBI) 51691
RRIDAB_513438
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing[PMID:15075370]. Lsm2-Lsm8 (Lsm stands for Like Sm), binds and stabilizes the 3′ end of the spliceosomal U6 snRNA Lsm proteins facilitate the annealing of U6 snRNA with U4 snRNA in vitro and interact with the U4/U6 annealing factor Prp24, suggesting that Lsm proteins function in U4/U6 snRNP formation in vivo [PMID:15485930, 17251193].

Protocols

Product Specific Protocols
WB protocol for LSM8 antibody 10134-1-APDownload protocol
IHC protocol for LSM8 antibody 10134-1-APDownload protocol
IF protocol for LSM8 antibody 10134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.

Authors - Xin Wang
View Article