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LSM4 Polyclonal antibody

LSM4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10834-1-AP

Synonyms

YER112W, U6 snRNA-associated Sm-like protein LSm4, GRP, Glycine-rich protein, Glycine rich protein

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using LSM4 Polyclonal antibody (10834-1-AP)

    WB analysis of MCF-7 using 10834-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10834-1-AP (LSM4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using LSM4 Polyclonal antibody (10834-1-AP)

    WB analysis of human placenta using 10834-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10834-1-AP (LSM4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-231 cells using LSM4 Polyclonal antibody (10834-1-AP)

    WB analysis of MDA-MB-231 using 10834-1-AP

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 10834-1-AP (LSM4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LSM4 Polyclonal antibody (10834-1-AP)

    IHC staining of human lung cancer using 10834-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10834-1-AP (LSM4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LSM4 Polyclonal antibody (10834-1-AP)

    IHC staining of human lung cancer using 10834-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10834-1-AP (LSM4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using LSM4 Polyclonal antibody (10834-1-AP)

    IF Staining of MCF-7 using 10834-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 10834-1-AP (LSM4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF7 cells, human placenta tissue, MDA-MB-231 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

10834-1-AP targets LSM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSM4 fusion protein Ag1280
Full Name LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC022198
Gene Symbol LSM4
Gene ID (NCBI) 25804
RRIDAB_2138361
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sm-like proteins, such as LSM4, were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing

Protocols

Product Specific Protocols
WB protocol for LSM4 antibody 10834-1-APDownload protocol
IHC protocol for LSM4 antibody 10834-1-APDownload protocol
IF protocol for LSM4 antibody 10834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

EDC3 phosphorylation regulates growth and invasion through controlling P-body formation and dynamics.

Authors - Jeremiah J Bearss
View Article
humanWB

Mol Cell Biol

A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis.

Authors - Berg Michael G MG
View Article