LSM14A Polyclonal antibody
LSM14A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 18336-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, PC-3 cells, HEK-293T cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IF | See 8 publications below |
| CoIP | See 1 publications below |
Product Information
18336-1-AP targets LSM14A in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LSM14A fusion protein Ag12997 |
| Full Name | LSM14A, SCD6 homolog A (S. cerevisiae) |
| Calculated Molecular Weight | 463 aa, 51 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC016842 |
| Gene Symbol | LSM14A |
| Gene ID (NCBI) | 26065 |
| RRID | AB_10644339 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LSm14A was originally discovered to be a component of the P-bodies, it is a member of the LSm family of proteins, which have been shown to be involved in RNA processing events. The N terminus of LSm14A is a conserved LSm domain, whereas the C terminus contains two domains called "DFDF box" and "FDF_TFG box", respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LSM14A antibody 18336-1-AP | Download protocol |
| IHC protocol for LSM14A antibody 18336-1-AP | Download protocol |
| IF protocol for LSM14A antibody 18336-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition | ||
Cell Rep The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation | ||
Adv Sci (Weinh) Oleic Acid Dissolves cGAS-DNA Phase Separation to Inhibit Immune Surveillance | ||
Nucleic Acids Res RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules | ||
