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LRWD1 Polyclonal antibody

LRWD1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 19546-1-AP

Synonyms

LRWD1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LRWD1 Polyclonal antibody (19546-1-AP)

    WB analysis using 19546-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19546-1-AP (LRWD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LRWD1 Polyclonal antibody (19546-1-AP)

    WB analysis using 19546-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19546-1-AP (LRWD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using LRWD1 Polyclonal antibody (19546-1-AP)

    IP experiment of mouse testis using 19546-1-AP

    IP result of anti-LRWD1 (IP:19546-1-AP, 4ug; Detection:19546-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

19546-1-AP targets LRWD1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name leucine-rich repeats and WD repeat domain containing 1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberNM_152892
Gene Symbol LRWD1
Gene ID (NCBI) 222229
RRIDAB_2878588
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRWD1 contains 3 LRR (leucine-rich) repeats and 5 WD repeats. LRWD1 is phosphorylated upon DNA damage by ATM or ATR. The antibody recognize the N-term of LRWD1.

Protocols

Product Specific Protocols
WB protocol for LRWD1 antibody 19546-1-APDownload protocol
IP protocol for LRWD1 antibody 19546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Development

PRSS50 is a testis protease responsible for proper sperm tail formation and function.

Authors - Jason M Scovell
View Article