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  • Featured Product
  • KD/KO Validated

LRSAM1 Polyclonal antibody

LRSAM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24666-1-AP

Synonyms

RING-type E3 ubiquitin transferase LRSAM1, RIFLE, Leucine-rich repeat and sterile alpha motif-containing protein 1, hTAL, EC:2.3.2.27

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using LRSAM1 Polyclonal antibody (24666-1-AP)

    WB analysis of Jurkat using 24666-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using LRSAM1 Polyclonal antibody (24666-1-AP)

    WB analysis of HepG2 using 24666-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using LRSAM1 Polyclonal antibody (24666-1-AP)

    WB analysis of mouse liver using 24666-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using LRSAM1 Polyclonal antibody (24666-1-AP)

    IHC staining of human skeletal muscle using 24666-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24666-1-AP (LRSAM1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using LRSAM1 Polyclonal antibody (24666-1-AP)

    IHC staining of human skeletal muscle using 24666-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24666-1-AP (LRSAM1 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using LRSAM1 Polyclonal antibody (24666-1-AP)

    IF Staining of A431 using 24666-1-AP

    Immunofluorescent analysis of A431 cells using 24666-1-AP (LRSAM1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, mouse liver tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24666-1-AP targets LRSAM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRSAM1 fusion protein Ag20415
Full Name leucine rich repeat and sterile alpha motif containing 1
Calculated Molecular Weight 723 aa, 84 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC009239
Gene Symbol LRSAM1
Gene ID (NCBI) 90678
RRIDAB_2879665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRSAM1 also named as hTAL, RIFLE orTsg101 associated ligase is a 723 amino acid protein, which contains 6 LRR repeats, 1 Ring-type zinc finger and 1 SAM domain. LRSAM1 is localizes in cytoplasm and highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue. As a E3 ubiquitin protein ligase, LRSAM1 mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic and exocytic cargos, LRSAM1 gene mutation has an association with Charcot Maire Tooth disease 2P.

Protocols

Product Specific Protocols
WB protocol for LRSAM1 antibody 24666-1-APDownload protocol
IHC protocol for LRSAM1 antibody 24666-1-APDownload protocol
IF protocol for LRSAM1 antibody 24666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules

Authors - Qinqin Cui
View Article
humanWB

Cancer Cell Int

Leucine-rich repeat and sterile alpha motif containing 1 promotes the oncogenic growth of human hepatocellular carcinoma cells.

Authors - Lili Pian
  • KD Validated
View Article
humanWB,IF

Autophagy

TRIM16-mediated lysophagy suppresses high-glucose-accumulated neuronal Aβ

Authors - Chang Woo Chae
View Article