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LRRC59 Polyclonal antibody

LRRC59 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27208-1-AP

Synonyms

LRRC59, PRO1855

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using LRRC59 Polyclonal antibody (27208-1-AP)

    WB analysis using 27208-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27208-1-AP (LRRC59 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using LRRC59 Polyclonal antibody (27208-1-AP)

    IHC staining of human colon using 27208-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27208-1-AP (LRRC59 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using LRRC59 Polyclonal antibody (27208-1-AP)

    IHC staining of human colon using 27208-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27208-1-AP (LRRC59 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27208-1-AP targets LRRC59 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRC59 fusion protein Ag26090
Full Name leucine rich repeat containing 59
Calculated Molecular Weight 307 aa, 35 kDa
Observed Molecular Weight 35-45 kDa
GenBank Accession NumberBC052279
Gene Symbol LRRC59
Gene ID (NCBI) 55379
RRIDAB_2880801
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LRRC59 antibody 27208-1-APDownload protocol
IHC protocol for LRRC59 antibody 27208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

OrthoID: profiling dynamic proteomes through time and space using mutually orthogonal chemical tools

Authors - Ara Lee
View Article