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LRRC41 Polyclonal antibody

LRRC41 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20457-1-AP

Synonyms

Protein Muf1, PP7759, MUF1, Leucine-rich repeat-containing protein 41

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using LRRC41 Polyclonal antibody (20457-1-AP)

    WB analysis of human brain using 20457-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20457-1-AP (LRRC41 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using LRRC41 Polyclonal antibody (20457-1-AP)

    IP experiment of K-562 using 20457-1-AP

    IP result of anti-LRRC41 (IP:20457-1-AP, 4ug; Detection:20457-1-AP 1:300) with K-562 cells lysate 1820 ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using LRRC41 Polyclonal antibody (20457-1-AP)

    IHC staining of human testis using 20457-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20457-1-AP (LRRC41 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LRRC41 Polyclonal antibody (20457-1-AP)

    IHC staining of human testis using 20457-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20457-1-AP (LRRC41 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using LRRC41 Polyclonal antibody (20457-1-AP)

    IHC staining of human liver using 20457-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 20457-1-AP (LRRC41 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using LRRC41 Polyclonal antibody (20457-1-AP)

    IHC staining of human liver using 20457-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 20457-1-AP (LRRC41 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20457-1-AP targets LRRC41 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRC41 fusion protein Ag14266
Full Name leucine rich repeat containing 41
Calculated Molecular Weight 812 aa, 87 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC004953
Gene Symbol LRRC41
Gene ID (NCBI) 10489
RRIDAB_10858385
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LRRC41 antibody 20457-1-APDownload protocol
IHC protocol for LRRC41 antibody 20457-1-APDownload protocol
IP protocol for LRRC41 antibody 20457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols