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  • Featured Product
  • KD/KO Validated

LRPPRC Polyclonal antibody

LRPPRC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 21175-1-AP

Synonyms

LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 130 kDa leucine-rich protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LRPPRC Polyclonal antibody (21175-1-AP)

    WB analysis using 21175-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LRPPRC Polyclonal antibody (21175-1-AP)

    WB analysis using 21175-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using LRPPRC Polyclonal antibody (21175-1-AP)

    WB analysis of rat kidney using 21175-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using LRPPRC Polyclonal antibody (21175-1-AP)

    WB analysis of rat liver using 21175-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using LRPPRC Polyclonal antibody (21175-1-AP)

    WB analysis of mouse heart using 21175-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using LRPPRC Polyclonal antibody (21175-1-AP)

    WB analysis of HepG2 using 21175-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using LRPPRC Polyclonal antibody (21175-1-AP)

    WB analysis of mouse skeletal muscle using 21175-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using LRPPRC Polyclonal antibody (21175-1-AP)

    WB analysis of mouse kidney using 21175-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using LRPPRC Polyclonal antibody (21175-1-AP)

    WB analysis of mouse liver using 21175-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LRPPRC Polyclonal antibody (21175-1-AP)

    WB analysis of HeLa using 21175-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using LRPPRC Polyclonal antibody (21175-1-AP)

    IP experiment of HeLa using 21175-1-AP

    IP result of anti-LRPPRC (IP:21175-1-AP, 5ug; Detection:21175-1-AP 1:1000) with HeLa cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LRPPRC Polyclonal antibody (21175-1-AP)

    IHC staining of human kidney using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LRPPRC Polyclonal antibody (21175-1-AP)

    IHC staining of human kidney using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using LRPPRC Polyclonal antibody (21175-1-AP)

    IHC staining of human normal colon using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using LRPPRC Polyclonal antibody (21175-1-AP)

    IHC staining of human heart using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21175-1-AP (LRPPRC antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using LRPPRC Polyclonal antibody (21175-1-AP)

    IHC staining of human heart using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21175-1-AP (LRPPRC antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human normal colon using LRPPRC Polyclonal antibody (21175-1-AP)

    IHC staining of human normal colon using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using LRPPRC Polyclonal antibody (21175-1-AP)

    IF Staining of U2OS using 21175-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 21175-1-AP (LRPPRC antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using LRPPRC Polyclonal antibody (21175-1-AP)

    IF Staining of HEK-293 using 21175-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using LRPPRC antibody (21175-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LRPPRC Polyclonal antibody (21175-1-AP)

    IF Staining of HepG2 using 21175-1-AP

    Immunofluorescent analysis of HepG2 cells, using LRPPRC antibody 21175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LRPPRC Polyclonal antibody (21175-1-AP)

    IF Staining of HeLa using 21175-1-AP

    Immunofluorescent analysis of Hela cells, using LRPPRC antibody 21175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using LRPPRC Polyclonal antibody (21175-1-AP)

    IF Staining of HEK-293 using 21175-1-AP

    Immunofluorescent analysis of 293 cells, using LRPPRC antibody 21175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using LRPPRC Polyclonal antibody (21175-1-AP)

    IF Staining of U2OS using 21175-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 21175-1-AP (LRPPRC antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using LRPPRC Polyclonal antibody (21175-1-AP)

    IF Staining of HEK-293 using 21175-1-AP

    Immunofluorescent analysis of HEK-293 cells using 21175-1-AP (LRPPRC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHCT 116 cells, mouse heart tissue, HepG2 cells, mouse skeletal muscle tissue, mouse kidney tissue, mouse liver tissue, HeLa cells, rat kidney tissue, rat liver tissue, rat heart tissue, PC-12 cells, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human heart tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HepG2 cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21175-1-AP targets LRPPRC in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRPPRC fusion protein Ag15452
Full Name leucine-rich PPR-motif containing
Calculated Molecular Weight 1394 aa, 158 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC050311
Gene Symbol LRPPRC
Gene ID (NCBI) 10128
RRIDAB_10733879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

Protocols

Product Specific Protocols
WB protocol for LRPPRC antibody 21175-1-APDownload protocol
IHC protocol for LRPPRC antibody 21175-1-APDownload protocol
IF protocol for LRPPRC antibody 21175-1-APDownload protocol
IP protocol for LRPPRC antibody 21175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIP

Nat Biotechnol

Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames.

Authors - Xue-Hai Liang
View Article
mouseIF,WB,CoIP

Nat Commun

Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA

Authors - Ayesha Sen
View Article
humanWB,IF

Nat Commun

A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.

Authors - Peng Sun
View Article
humanWB

Genome Biol

Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes

Authors - Iliana Soto
  • KO Validated
View Article
humanIHC,CoIP

Redox Biol

LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.

Authors - Wen-Su Wei
View Article
humanWB

EMBO J

Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells.

Authors - Yanni Ma
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Angela (Verified Customer) (10-09-2018)

Worked well with 20 ug whole cell lysate run on 7% Tris-Acetate SDS-PAGE. Clean band seen between 120-150 kDa.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:6000
  • Cell Tissue Type: HEK293 cells